restriction endonuclease वाक्य
उदाहरण वाक्य
मोबाइल
- Restriction Endonuclease enzymes play a very important role in modern molecular cloning techniques.
- A restriction endonuclease functions by " scanning " the length of a DNA molecule.
- An example of a Type I restriction endonuclease.
- However, if genomic DNA is treated with a particular restriction endonuclease many DSBs will be created.
- Most commonly used restriction enzymes are Type II restriction endonuclease ( See article on Restriction enzymes for examples ).
- The plasmid contains a genetic sequence corresponding to the recognition site of a restriction endonuclease, such as EcoR1.
- Sickle-cell gene mutation probably arose spontaneously in different geographic areas, as suggested by restriction endonuclease analysis.
- When examining the preferential double strand cleavage of DNA, the restriction endonuclease PstI bind to pSM1 plasmid DNA.
- The sequence altered by the mutation eliminates the recognition site for the restriction endonuclease MstII that recognizes the nucleotide sequence.
- Ronald W . Davis first discovered sticky ends as the product of the action of EcoRI, the restriction endonuclease.
- EcoRV is the only type II restriction endonuclease known to cause a major protein-induced conformational change in the DNA.
- The cloning vector is treated with a restriction endonuclease to cleave the DNA at the site where foreign DNA will be inserted.
- A D86G mutation of " EMG1 " destroys an EcoRV restriction endonuclease site in the most highly conserved region of the protein.
- In the case of large DNA molecules, the DNA is frequently cut into smaller fragments using a DNA restriction endonuclease ( or restriction enzyme ).
- A restriction endonuclease typically requires a recognition site and a cleavage pattern ( typically of nucleotide bases : A, C, G, T ).
- The crystal structure of Prp8 protein ( residues 885 2413 ) reveals tightly associated domains that resemble an intron reverse transcriptase and a type II restriction endonuclease.
- DNA samples before or after restriction enzyme ( restriction endonuclease ) digestion are separated by gel electrophoresis and then transferred to a membrane by blotting via capillary action.
- The positions of these two cuts, both in relation to each other, and to the recognition sequence itself, are determined by the identity of the restriction endonuclease.
- 19 . Haertl R, Bandlow G . Epidemiological fingerprinting of Enterobacter cloacae by small-fragment restriction endonuclease analysis and pulsed-field gel electrophoresis of genomic restriction fragments.
- A Restriction endonuclease enzyme is extracted from Alu that acts at the centre of a palindromic tetranucleotide sequence to give even-ended duplex DNA fragments phosphorylated at the 5'- end.
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